BioSim™ anti-Daratumumab (Human) ELISA Kit

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SKU:
26-E4924
Availability:
Usually Shipped in 5 Working Days
Size:
96 Assays
Storage Conditions:
4ºC
Shipping Conditions:
Gel Pack
Shelf life:
12 months
$1,855.20
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Description

Daratumumab is a human IgG1 monoclonal antibody that binds with a high affinity to a unique epitope of CD38. CD38 is a type II transmembrane glycoprotein expressed on lymphoid, non-hematopoietic tissues, and myeloid cells. CD38 possesses ectoenzyme activity and plays a significant role in receptor-mediated regulation of cell adhesion and signal transduction. It is expressed at high levels on multiple myeloma cells, thus making it a unique target for therapeutic antibodies targeting multiple myeloma cell surfaces. The monoclonal antibody binds to CD38 on the surface of multiple myeloma cells and induces cell death via multiple mechanisms: complement-dependent cytotoxicity (CDC), antibody-dependent cell-mediated cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), and apoptosis. The original antibody is approved to treat multiple myeloma in patients who have received at least 3 previous therapies. However, upon administration of the antibody, patients tend to develop an immune response that results in the formation of anti-Daratumumab antibodies, thus adversely affecting the therapeutic response to treatment. BioSim™ anti-Daratumumab ELISA kit qualitatively measures antibodies to Daratumumab in human serum or plasma samples. The kit is based on the Sandwich ELISA principle. Standards and samples (serum or plasma) are added to the microtiter plate coated with Daratumumab. After incubation, the wells are washed. The HRP conjugated probe is added and binds to the antibodies to Daratumumab that is captured by Daratumumab coated on the wells. Following incubation, wells are washed and the enzymatic activity is detected by the addition of TMB chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of antibodies to Daratumumab in the sample or standard. The qualitative test results can be evaluated using cut-off values.

Alternate Name: ADPRC1, ADPRC 1, CD38 Molecule, T10, CD38, EC 3.2.2.6, EC 2.4.99.20, CADPr Hydrolase 1, Cyclic ADP-Ribose Hydrolase 1, ADP-Ribosyl Cyclase 1, CAS 945721-28-8

Tag Line: A Sandwich ELISA kit for the Qualitative measurement of antibodies to Daratumumab in human serum or plasma samples

Summary: N/A

Detection Method: Absorbance (450 nm)

Sample Type: Plasma,Serum

Species Reactivity: Human

Applications: This Sandwich ELISA kit is used for in vitro Qualitative determination of antibodies to Daratumumab in human serum and plasma samples

Features & Benefits: Cross Reactivity: Except for Daratumumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins,For in vitro Qualitative determination of antibodies to Daratumumab in human serum and plasma samples,Assay Precision: Intra-Assay and Inter-Assay CV < 30%

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