Human CellExp™ MMP-9, human recombinant

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SKU:
26-7246
Availability:
Usually Shipped in 5 Working Days
Storage Temperature:
-20°C
Shipping Conditions:
Gel Pack
Shelf Life:
12 months
zł1,749.56 - zł20,097.04
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Description

Biomolecule/Target: MMP-9

Synonyms: MMP9, CLG4B, GELB, MANDP2, MMP-9, Matrix Metalloproteinase-9

Alternates names: MMP9, CLG4B, GELB, MANDP2, MMP-9, Matrix Metalloproteinase-9

Taglines: Involved in the breakdown of extracellular matrix

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 4318

NCBI Gene Symbol: MMP-9

Gene Source: Human

Accession #: P14780

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).

Activity (Specifications/test method): N/A

Biological activity: Pre-activation is required for enzymatic assays using 20 mM APMA stock. Both activation and assay reaction can be done using BioVision’s MMP-9 Assay Buffer (25 ml) (Cat. No. K844-100-1).

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein has 450 amino acids with a 6x His tag at C-terminus, and a calculated MW of 51 kDa. The N-terminal is Ala 20. The protein migrates to ~60 kDa under reducing conditions (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from 0.22 μm filtered PBS (pH 7.4) with 5% trehalose.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.

Background Information: Matrix metallopeptidase 9 (MMP-9) is secreted from neutrophils, macrophages and a number of transformed cells. It is the most complex MMP family member in terms of domain structure and regulation of its activity. MMP family is involved in the breakdown of extracellular matrix in normal physiological processes as well as in disease processes. Most MMPs are secreted as inactive pro-proteins which are activated by the cleavage of extracellular proteinases. MMP-9 degrades various substrates including gelatin, collagen types IV and V, and elastin. It is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and diseases including systemic lupus erythematosus, rheumatoid arthritis, and multiple sclerosis. MMP-9 is regarded as a potential therapeutic target and a biomarker of disease status.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans

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