OrgFrontier™ Peroxisome Isolation Kit

Peroxisomes are small, single membrane-bound organelles that are found in all eukaryotic cells and are similar to lysosomes in size and morphology. Peroxisomes contain a variety of enzymes that utilize molecular oxygen to perform oxidation reactions. Peroxisomal enzymes are involved in the oxidation of ethanol, long-chain fatty acids, branched chain fatty acids, D-amino acids, and polyamines. Hydrogen peroxide, a product of the oxidative enzymatic reaction is subsequently converted to water and oxygen by catalase, an abundant peroxisomal enzyme. Peroxisomes are also involved in the biosynthesis of ether phospholipids known as plasmalogens that are required for the myelination of neurons. BioVision’s OrgFrontier™ Peroxisome Isolation Kit provides a proprietary set of reagents and buffers designed to enable the extraction and isolation of intact peroxisomes from mammalian tissues and cultured cells. The protocol requires an ultracentrifugation step and can be completed in one day.

Alternate Name: OrgFrontier™ Peroxisome Isolation Kit

Tag Line: For extraction and isolation of intact peroxisomes

Summary: BioVision’s OrgFrontier™ Peroxisome Isolation Kit provides a proprietary set of reagents and buffers designed to enable the extraction and isolation of intact peroxisomes from mammalian tissues and cultured cells. The protocol requires an ultracentrifugation step and can be completed in one day.

Detection Method: Gradient Separation

Sample Type: Cultured mammalian cells and mammalian tissues

Species Reactivity: Mammalian

Applications: Enrichment of peroxisome-associated proteins for western blot and ELISA, Peroxisome studies and peroxisome protein profiling, Isolation of peroxisomes from mammalian tissues and cultured cells

Features & Benefits: Extraction and isolation of intact peroxisomes from mammalian tissues and cultured cells.