PicoProbe™ Threonine Assay Kit (Fluorometric)

Threonine is one of the 20 naturally-occuring amino acids used by all organisms in the biosynthesis of proteins and is an essential dietary amino acid for humans. Threonine residues in proteins are subject to post-translational phosphorylation, a critical regulatory and signal transduction mechanism. In addition to its role as a protein building block, threonine can serve as a precursor to other amino acids and metabolites, including glycine, isoleucine and acetyl-coenzyme A. Recent research has shown that threonine is a vital nutrient for embryonic stem cells and threonine metabolism (mediated by the enzyme threonine dehydrogenase) is required for unlimited self-renewal and maintenance of stem cell pluripotency. Dietary threonine deficiency, while rare, can result in breakdown of the intestinal mucosal lining (eventually triggering inflammatory bowel disorders), immunosuppression and a multitude of neuropsychiatric symptoms. BioVision’s Threonine Assay Kit allows for highly sensitive quantification of L-Threonine levels in biological fluids and tissues. The assay is based on the selective, NAD+-coupled enzymatic metabolism of threonine, yielding an oxidized intermediate and NADH. A developer enzyme mixture utilizes the NADH generated to convert PicoProbe™ into a stable fluorophore (Ex/Em = 538/587 nm). The assay is not affected by physiological concentrations of other amino acids, is high-throughput adaptable and can detect threonine levels down to 2 μM in samples

Alternate Name: N/A

Tag Line: Market's first and only kit for enzymatic detection of Threonine in biological samples

Summary: • Detection method- Fluorescence (Ex/Em 535/587 nm) • Application- Measurement of Threonine in biological samples such as serum, plasma, CSF, etc.

Detection Method: Fluorescence (Ex/Em: 535/587 nm)

Sample Type: • Human or animal biological fluids (plasma, serum, CSF, etc.) • Soft tissue homogenates (i.e. liver, brain, etc.) • Cultured cell lysates (adherent or suspension cells) or cell culture growth medium

Species Reactivity: Mammalian

Applications: Estimation of Threonine in various biological samples

Features & Benefits: • Simple, rapid & convenient assay • Limit of detection: 2 µM • Very specific, does not interact with other standard and non-standard amino acids