ABTS™ (solid)

ABTS is considered a safe and sensitive substrate compatible for horseradish peroxidase (HRP) based ELISA assays. In the presence of hydrogen peroxide and HRP, ABTS is oxidized to a radical cation with an absorption maxima at 820 nm, 734 nm, 650 nm and 405 nm. The latter frequency demonstrates a significant molar extinction coefficient and is generally employed for most ABTS assays. Stopping the reaction with acid does not alter the 405 nm spectrum allowing kinetic and endpoint methods to be measured at the same wavelength.

Alternate Name/Synonyms: 2,2'-(1,2-hydrazinediylidene)bis[3-ethyl-2,3-dihydro-6-benzothiazolesulfonic acid, diammonium salt

Appearance: Light green powder

Appearance:

Appearance: 30931-67-0

Appearance: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₈H₂₄N₆O₆S₄

Molecular Weight: 548.68

Cell-Permeable?: FALSE

Purity: ≥98% by HPLC

Solubilities: ~10 mg/ml, DMSO >40 mg/ml Water

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A peroxidase substrate

MDL Number: MFCD00010404

PubChem CID: 9570474

SMILES: [O-]S(C1=CC=C(N(CC)/C(S2)=N/N=C3SC(C=C(S([O-])(=O)=O)C=C4)=C4N\3CC)C2=C1)(=O)=O.[NH4+].[NH4+]

InChi: InChI=1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

InChi Key: OHDRQQURAXLVGJ-AXMZSLBLSA-N

Category: Biochemicals