ABTS™ Solution

ABTS is considered a safe and sensitive substrate compatible for horseradish peroxidase (HRP) based ELISA assays. In the presence of hydrogen peroxide and HRP, ABTS is oxidized to a radical cation with an adsorption maxima at 820 nm, 734 nm, 650 nm and 405 nm. The latter frequency demonstrates a significant molar extinction coefficient and is generally employed for most ABTS assays. Stopping the reaction with acid does not alter the 405 nm spectrum allowing kinetic and endpoint methods to be measured at the same wavelength. Using a proprietary-nontoxic stabilization technology, BioVision, Inc. provides a room temperature stable, single component ABTS solution for quantitative analysis of HRP based systems.

Alternate Name/Synonyms: 2,2'-Azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid)

Appearance: Liquid

Appearance: 1.46 mMol L⁻¹, in a citrate buffer, pH 4.0.

Appearance: 28752-68-3

Appearance: No

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₈H₁₈N₄O₆S₄

Molecular Weight: 514.6

Cell-Permeable?: No

Purity: N/A

Solubilities: N/A

Handling: Protect from light

Country of Origin: USA

Tag Line: A peroxidase substrate

MDL Number: MFCD00214141

PubChem CID: 35688

SMILES: CCN1C2=C(C=C(C=C2)S(=O)(=O)O)SC1=NN=C3N(C4=C(S3)C=C(C=C4)S(=O)(=O)O)CC

InChi: InChI=1S/C18H18N4O6S4/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26, 27)28)10-16(14)30-18/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28)

InChi Key: ZTOJFFHGPLIVKCUHFFFAOYSA-N

Category: Biochemicals