OxiRed™ Probe

OxiRed, which has been marketed under the name Amplex Red™ reagent, is a highly sensitive and stable probe for H₂O₂, is a fluorogenic (EX/EM = 535/587 nm) and Colorometric (l = 570 nm) substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the OxiRed probe reacts in a 1:1 stoichiometry with H₂O2 to produce highly fluorescent resorufin. Because H₂O₂ is produced in many different enzymatic reactions, the OxiRed probe allows researchers to detect the activity of many different enzymes either colorimetrically or fluorometrically.

Alternate Name/Synonyms: 10-acetyl-3,7-dihydroxyphenoxazine

Appearance: White to pink solid

Appearance: N/A

Appearance: 119171-73-2

Appearance: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₄H₁₁NO₄

Molecular Weight: 257.25

Cell-Permeable?: Yes

Purity: ≥99%

Solubilities: DMSO or DMF

Handling: Protect from light and air

Country of Origin: USA

Tag Line: A hydrogen peroxide probe

MDL Number: MFCD00467861

PubChem CID: 167453

SMILES: CC(=O)N1C2=C(C=C(C=C2)O)OC3=C1C=CC(=C3)O

InChi: InChI=1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12 (14)15/h2-7,17-18H,1H3

InChi Key: PKYCWFICOKSIHZ-UHFFFAOYSA-N

Category: Biochemicals