EZCut™ SUMO Protease 1 (GST-tagged), Yeast Recombinant

Biomolecule/Target: SUMO Protease 1

Synonyms: Ulp1 peptidase, SUMO Protease

Alternates names: Ulp1 peptidase, SUMO Protease

Taglines: >90% Pure, Active, Yeast Recombinant, GST-Tagged SUMO Protease 1, to cleave the SUMO tag from recombinant proteins.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 856087

NCBI Gene Symbol: ULP1

Gene Source: Yeast

Accession #: Q02724

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGE #: ≥90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): The EZCut™ SUMO Protease 1 (GST-tagged) has an activity of ≥10,000 units/mg.

Biological activity: N/A

Results: ≥10,000 units/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of EZCut™ SUMO Protease 1 required to cleave >90% of 5 µg a control protein substrate (SUMO-GFP) in 1 h at 37°C.

Molecular Weight: 52.6 kDa (403-621 aa + N-terminal GST).

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT and 20% glycerol pH 8.0

Reconstitution Instructions: N/A

Background Information: SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. It is highly specific as it recognizes the tertiary structure of the ubiquitin-like (UBL) protein, SUMO (Smt3), rather than its amino acid sequence. SUMO fusion tag, as an N-terminal fusion partner, has been shown to enhance functional protein production in prokaryotic and eukaryotic expression systems with significantly improved protein stability and solubility. BioVision’s Recombinant EZCut™ SUMO Protease 1 containing an N-terminal GST-tag is expressed in E. coli and purified by proprietary chromatographic techniques. The EZCut™ SUMO protease 1 can be used to cleave SUMO protein tag from recombinant SUMO-fusion proteins. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH. After the completion of the cleavage reaction, the protease can be easily removed from the reaction by affinity chromatography using the Glutathione resin (Cat. # 6555-1,10,50).

Amino acid sequence: 403-621 aa + N-terminal GST

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans