Human CellExp™ SARS-CoV-2 Spike RBD (L452R, T478K), Recombinant

Biomolecule/Target: N/A

Synonyms: COVID-19 Spike RBD protein; 2019-nCoV Spike RBD protein; SARS-CoV-2 Mutant (L452R, T478K)

Alternates names: N/A

Taglines: Recombinant Spike RBD mutant of the SARS-CoV-2 Delta variant

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 43740568

NCBI Gene Symbol: N/A

Gene Source: SARS-CoV-2

Accession #: P0DTC2

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥ 90% by SDS-PAGE

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1 EU per 1 µg of protein as determined by LAL method

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 26.6 kDa (calculated); 33-35 kDa (observed due to glycosylation)

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized powder

Reconstitution Instructions: N/A

Background Information: SARS-CoV-2 Spike protein is a large type I transmembrane protein composed of S1 subunit and S2 subunit. During viral infection, the receptor-binding domain (RBD) of the S1 subunit is responsible for the recognition and binding of host receptor ACE2, while the S2 subunit mediates viral cell membrane fusion. The SARS-CoV-2-S1-RBD/ACE2 interaction mediates viral entry into the target cells. The mutations at L452R and T478K were identified in the SARS-CoV-2 Delta (particularly B.1.617.2) variants that originated in India.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans