Human CellExp™ Carbonic Anhydrase 4/CA4, human recombinant

Biomolecule/Target: Carbonic Anhydrase 4/CA4

Synonyms: CA4, Carbonic anhydrase 4

Alternates names: CA4, Carbonic anhydrase 4

Taglines: May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 762

NCBI Gene Symbol: CA4

Gene Source: Human

Accession #: P22748

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥92%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its esterase activity. The specific activity is >2 pmol/min/µg.

Results: Measured by its esterase activity. The specific activity is >2 pmol/min/µg.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 31 kDa. The predicted N-terminus is Ala 19. DTT-reduced Protein migrates as 33 kDa in SDS-PAGE.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Carbonic anhydrase 4 (CA4) is also known as Carbonate dehydratase IV, Carbonic anhydrase IV, which belongs to the alpha-carbonic anhydrase family. CA4 may stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. The enzyme regulation is activated by histamine, L-adrenaline, D-phenylalanine, L- and D-histidine. CA4 can interact with SLC4A4.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans