Human CellExp™ CD27 Ligand/ CD70, Fc Tag, Mouse Recombinant

Biomolecule/Target: CD70

Synonyms: CD70, CD27LG, TNFSF7, TNFSF7G, CD27L

Alternates names: CD70, CD27LG, TNFSF7, TNFSF7G, CD27L

Taglines: Cytokine that binds to CD27 and plays role in T-cell activation.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 21948

NCBI Gene Symbol: CD70

Gene Source: Mouse

Accession #: O55237

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: >95%

Assay: SDS-PAGE

Purity: >95%

Assay #2: HPLC

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA

Biological activity: Immobilized Mouse CD27, Mouse IgG2a Fc Tag, at 2 μg/mL (100 μL/well) can bind Mouse CD27 Ligand (active trimer) with a linear range of 0.1-4 ng/mL

Results: N/A

Binding Capacity: Immobilized Mouse CD27, Mouse IgG2a Fc Tag, at 2 μg/mL (100 μL/well) can bind Mouse CD27 Ligand (active trimer) with a linear range of 0.1-4 ng/mL

Unit Definition: N/A

Molecular Weight: 18.6 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to a concentration of 100 µg/ml.

Background Information: Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used on humans