Human CellExp™ CD28, Human recombinant

Biomolecule/Target: CD28

Synonyms: CD28, Tp44

Alternates names: CD28, Tp44

Taglines: T-cell-specific surface glycoprotein CD28

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 940

NCBI Gene Symbol: CD28

Gene Source: Human

Accession #: AAH93698

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥92%

Assay: SDS-PAGE

Purity: ≥92%

Assay #2: N/A

Endotoxin Level: < 1 EU/μg

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Immobilized human CD28 at 2 μg/mL (100 μL/well) can bind human Biotin-B7-1 (CD80) Fc with a linear range of 0.01-0.5 μg/mL when detected by Streptavidin-HRP second antibody.

Results: N/A

Binding Capacity: Immobilized Human B7-1, His Tag at 20μg/ml, can bind Human CD28, Fc Tag with a linear of 0.2-20 μg/ml and Human B7-2, His Tag at 2μg/ml, can bind Human CD28, Fc Tag with a linear of 0.01-5 μg/ml

Unit Definition: N/A

Molecular Weight: This protein is fused with a Fc tag at C-terminus and has a calculated MW of 41.8 kDa.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Reconstitution Instructions: Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml

Background Information: T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans