Human CellExp™ CD36, human recombinant

Biomolecule/Target: CD36

Synonyms: CD36, SCARB3, GP3B, GP4, Platelet Glycoprotein 4

Alternates names: CD36, SCARB3, GP3B, GP4, Platelet Glycoprotein 4

Taglines: A multiligand pattern recognition receptor

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 948

NCBI Gene Symbol: CD36

Gene Source: Human

Accession #: P16671

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized rhCD36 at 2 μg/mL (100 μL/well) can bind rhTSP2/His with a linear range of 0.01-1 μg/mL

Results: Measured by its binding ability in a functional ELISA. Immobilized rhCD36 at 2 μg/mL (100 μL/well) can bind rhTSP2/His with a linear range of 0.01-1 μg/mL

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with C-terminal 6×his tag at C-terminus, has a calculated MW of 47.5 kDa expressed. The predicted N-terminus is Gly30. Protein migrates as 60-90 kDa in reduced SDS-PAGE resulting from glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 200 µg/ml. Solubilize for 30 to 60 min. at RT with occasional gentle mixing. Do not vortex. Carrier protein (0.1% HAS or BSA) is strongly recommended for further dilution and long term storage.

Background Information: CD36 (Cluster of Differentiation 36), also known as platelet membrane glycoprotein IV (GPIV), fatty acid translocase (FAT), thrombospondin receptor, collagen receptor, and scavenger receptor class B, member 3 (SRB3), is a member of the class B scavenger receptor family of cell surface proteins. The human CD36 gene encodes a single chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.CD36 is found on platelets, erythrocytes, monocytes, differentiated adipocytes, mammary epithelial cells, spleen cells and some skin microdermal endothelial cells. CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta-amyloid fibrils (fAβ), collagens I and IV, and Plasmodium falciparum infected erythrocytes. CD36 is required for the anti-angiogenic effects of thrombospondin1 in the corneal neovascularization assay. On binding a ligand the protein and ligand are internalized. This internalization is independent of macro pinocytosis and occurs by an actin dependent mechanism requiring the activation Src-family kinases, JNK and Rho-family GTPases. CD36 ligands have also been shown to promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans