null

Human CellExp™ GAD1, human recombinant

(No reviews yet) Write a Review
SKU:
26-7250
Availability:
Usually Shipped in 5 Working Days
Size:
10 μg
Storage Temperature:
-20°C
Shipping Conditions:
Gel Pack
Shelf Life:
12 months
€351.00
Frequently bought together:

Description

Biomolecule/Target: GAD1

Synonyms: GAD1, CPSQ1, GAD, SCP, GAD-1, CPSQ-1, GAD67, GAD-67

Alternates names: GAD1, CPSQ1, GAD, SCP, GAD-1, CPSQ-1, GAD67, GAD-67

Taglines: Serves as a highly prostate-specific tissue biomarker

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 2571

NCBI Gene Symbol: GAD1

Gene Source: Human

Accession #: Q99259

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥92%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×his tag at C-terminus, has a calculated MW of 67 kDa expressed. The predicted N-terminus is Met1. Protein migrates as the predominant 64 kDa form and a less-frequent 24-kDa form in reduced SDS-PAGE resulting from alternative splicing.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4, 1 mM EDTA with some stabilizer. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Glutamate decarboxylase 1 (GAD1), also known as 67 kDa glutamic acid decarboxylase and Glutamate decarboxylase 67 kDa isoform, is a member of the group II decarboxylase family. GAD1 is expressed in benign and malignant prostatic tissue and may serve as a highly prostate-specific tissue biomarker. GAD1 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. GAD1 may also play a role in the stiff man syndrome. Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) which is a non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. GAD1 has been shown to interact with GAD2. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans

View AllClose