Human CellExp™ IL-1beta, Human Recombinant
- Usually Shipped in 5 Working Days
- Storage Temperature:
- Shipping Conditions:
- gel pack
- Shelf Life:
- 12 months
Synonyms: Human Cellexp Human Recombinant IL-1beta
Alternates names: Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
Taglines: An important mediator of inflammatory responses
Country of Animal Origin: USA
NCBI Gene ID #.: 3553
NCBI Gene Symbol: IL1B
Gene Source: Human
Accession #: P01584
Source: Human 293 cell expressed
Purity by SDS-PAGE #: ≥95%
Assay #2: N/A
Endotoxin Level: < 1.0 EU per 1 μg of protein
Activity (Specifications/test method): N/A
Biological activity: ED₅₀ is typically 0.02 to 0.08 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells.
Results: 0.02 to 0.08 ng/ml
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 18 and 25 kDa, monomer, glycosylated
Physical form description: Lyophilized from a PBS solution.
Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Background Information: Interleukin-1beta is produced by activated macrophages. IL-1beta stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL1beta proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. In comparison with the E. coli expressed protein, IL-1 beta is 50% more potent in promoting human CD4+ T cell differentiation into Th17 cells. This cytokine is produced in a serumfree, chemically defined media.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans