Human CellExp™ IL-1beta, Human Recombinant

Biomolecule/Target: N/A

Synonyms: Human Cellexp Human Recombinant IL-1beta

Alternates names: Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.

Taglines: An important mediator of inflammatory responses

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 3553

NCBI Gene Symbol: IL1B

Gene Source: Human

Accession #: P01584

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 μg of protein

Activity (Specifications/test method): N/A

Biological activity: ED₅₀ is typically 0.02 to 0.08 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells.

Results: 0.02 to 0.08 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 18 and 25 kDa, monomer, glycosylated

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from a PBS solution.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Background Information: Interleukin-1beta is produced by activated macrophages. IL-1beta stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL1beta proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. In comparison with the E. coli expressed protein, IL-1 beta is 50% more potent in promoting human CD4+ T cell differentiation into Th17 cells. This cytokine is produced in a serumfree, chemically defined media.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans