Human CellExp™ Lipocalin-2 / LCN2, human recombinant

Biomolecule/Target: Lipocalin-2

Synonyms: NGAL, LCN2, Lipocalin-2, 24p3, MSFI, Siderocalin; Oncogene 24p3; Neutrophil Gelatinase-associated Lipocalin; NGAL.

Alternates names: NGAL, LCN2, Lipocalin-2, 24p3, MSFI, Siderocalin; Oncogene 24p3; Neutrophil Gelatinase-associated Lipocalin; NGAL.

Taglines: Functions as a growth factor and is upregulated during inflammation

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 3934

NCBI Gene Symbol: LCN2

Gene Source: Human

Accession #: P80188

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): Measured by its ability to bind Iron (III) dihydroxybenzoic acid. [Fe(DHBA)3]. The binding of Fe (DHBA)3 results in the quenching of Trp fluorescence in rhLCN2. rhLCN2 can bind >1.5 μM of Fe(DHBA)3.

Biological activity: Measured by its ability to bind Iron (III) dihydroxybenzoic acid. [Fe(DHBA)3]. The binding of Fe (DHBA)3 results in the quenching of Trp fluorescence in rhLCN2. rhLCN2 can bind >1.5 μM of Fe(DHBA)3.

Results: Measured by its ability to bind Iron (III) dihydroxybenzoic acid. [Fe(DHBA)3]. The binding of Fe (DHBA)3 results in the quenching of Trp fluorescence in rhLCN2. rhLCN2 can bind >1.5 μM of Fe(DHBA)3.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×his tag at C-terminal and has a calculated MW of 22 kDa. The predicted N-terminal is Gln 21. DTT-reduced protein migrates as 23-25 kDa.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Lipocalin-2 (LCN2), also known as oncogene 24p3 or neutrophil gelatinase-associated lipocalin (NGAL), MSFI, The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor. LCN2 is strongly upregulated during inflammation and is upregulated by interleukin 1 (but not TNF alpha) in humans. There are indications that some forms of acne could be caused due to the gene not being transcribed, and that Isotretinoin corrects this. NFAT3 (NFATc4) NFAT by blocking the expression of LCN2 inhibits breast carcinoma cell motility. Recent studies have revealed that NGAL plays an important role in the physiopathology of chronic myeloid leukaemia (CML) mediated by BCR-ABL.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans