Human CellExp™ SARS-CoV-2 Spike Protein (S1), Recombinant

Biomolecule/Target: N/A

Synonyms: S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1, novel coronavirus s1 Protein

Alternates names: S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1, novel coronavirus s1 Protein

Taglines: Glycoprotein that enables viral entry into the host cell

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 43740568

NCBI Gene Symbol: S

Gene Source: Coronavirus

Accession #: YP_009724390.1

Recombinant: TRUE

Source: HEK 293 cells

Purity by SDS-PAGE #: >90%

Assay: SDS - PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 Spike S1, the EC50 of SARS-COV-2 Spike S1 is 6.79 ng/mL.|Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 90.8 nM as determined in a SPR assay

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 103 kDa (Fc, 6×His tag at the C-terminus)

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Background Information: SARS-CoV is an enveloped, single, and positive-stranded RNA virus. Cell entry of severe acute respiratory syndrome coronavirus (SARS-CoV) is mediated by the viral spike (S) protein. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. For viral entry, the surface unit (S1) of SARS S binds to the cellular receptor angiotensin-converting enzyme 2 (ACE2) and the transmembrane unit (S2) then fuses the viral membrane with a host cell membrane. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV. Because the S protein of SARS-CoV is involved in receptor recognition, as well as virus attachment and entry, it represents one of the most important targets for the development of SARS vaccines and therapeutics.

Amino acid sequence: Val 11-Arg 682

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans