Human CellExp™ SERPIN A3, human recombinant

Biomolecule/Target: SERPIN A3

Synonyms: SERPINA3, ACT, AACT, GIG24, GIG25

Alternates names: SERPINA3, ACT, AACT, GIG24, GIG25

Taglines: Involved in inhibition of neutrophil cathepsin G and mast cell chymase

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 12

NCBI Gene Symbol: SERPINA3

Gene Source: Human

Accession #: P01011

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 value is <6 nM.

Results: Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 value is <6 nM.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 46 kDa. The predicted N-terminus is His 23. DTT-reduced Protein migrates as 55-70 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Serpin A3 is also known as Alpha-1-antichymotrypsin (ACT or AACT), Cell growth-inhibiting gene 24/25 protein, which belongs to the serpin family. Serpin A3 can bind DNA. Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans