CaspGLOW™ Fluorescein Active Caspase-3 Staining Kit

Activation of caspases plays a central role in apoptosis. The CaspGLOW™ Fluorescein Active Caspase-3 Staining Kit provides a convenient means for sensitive detection of activated caspase-3 in living cells. The assay utilizes the caspase-3 inhibitor, DEVD-FMK, conjugated to FITC (FITC-DEVD-FMK) as a marker. FITC-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells.

Alternate Name: N/A

Tag Line: Detects activated Caspase-3 by FACS, FL or plate reader

Summary: • Detection method- Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm) • Species reactivity- Mammalian • Kit size- Convenient sizes (25 and 100 assays) • Applications- Sensitive detection of activated caspase-3 in living cells.

Detection Method: N/A

Sample Type: Live cells

Species Reactivity: N/A

Applications: N/A

Features & Benefits: • Simple one-step procedure; takes only 1-2 hours • Fast and convenient • The FITC label allows for direct detection of the activated caspases in apoptotic cells