EZClick™ O-GlcNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Red)

Glycans are the vital components of glycoproteins, glycolipids, and proteoglycans. Glycoproteins are grouped by the type of carbohydrate and the amino acid linkage site. N-linked glycosylation is a modification of asparagine, whereas O-linked glycosylation occurs on the hydroxyl group of serine and threonine residues. Glycosylation occurs co- or post-translationally on more than 50% of eukaryotic proteins resulting in membrane-assisted, intracellular, or secreted glycoproteins that are crucial in cellular processes, protein bioactivity and metabolic turnover. Modification by O-linked-N-acetyl glucosamine (O-GlcNAc) has rapidly emerged as a major cellular signaling mechanism with number of modified targets similar to protein phosphorylation. O-GlcAc glycosylation is implicated in normal brain functions, etiology of neurodegeneration, type II diabetes, pathways involved in morphogenesis, and virulence factors of microbes in plant host cells. Since glycoproteins are not directly encoded in the genome, methods of characterization and analyses of glycoproteins are of great interest. BioVision’s EZClickTM O-GlcNAc Modified Glycoprotein Assay Kit offers a highly specific, simple and robust method for labeling and detection of O-GlcNAc modified glycoproteins. In this assay, a modified glucosamine precursor is fed directly into the cells, which is processed by the hexosamine pathway and is incorporated into proteins. This is followed by the click reaction with an alkyne-containing dye. This system offers a powerful method for imaging the localization, trafficking, dynamics of glycans, or detection by FACS for quantitative studies. Labeled Glycoproteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-alkyne or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.

Alternate Name: EZClick™ O-GlcNAc Modified Glycoprotein Assay Kit EZClick™ O-GlcNAc Modified Glycoprotein Kit

Tag Line: A highly specific, simple and robust assay for labeling and detection of O-GlcNAc modified glycoproteins.

Summary: BioVision’s EZClickTM O-GlcNAc Modified Glycoprotein Assay Kit offers a highly specific, simple and robust method for labeling and detection of O-GlcNAc modified glycoproteins.

Detection Method: FACS/Microscopy

Sample Type: Suspension or adherent cells

Species Reactivity: N/A

Applications: Identification and localization of O-GlcNAc-glycosylated proteins within cells. Evaluating effects of anti-cancer drugs and genotoxic agents on modified glycans. Screening for genotoxic compounds and effectors of modified glycans in proliferating cells. Detection and quantification of biosynthesis, dynamics and turnover of modified glycans. Imaging the localization, trafficking, and dynamics of modified glycans.

Features & Benefits: This system offers a powerful method for imaging the localization, trafficking, dynamics of glycans, or detection by FACS for quantitative studies. A highly specific, simple and robust method for labeling and detection of O-GlcNAc modified glycoproteins.