EZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

Palmitoylation occurs when fatty acids, such as Palmitic Acid are covalently attached to the side chains of cysteine (and less frequently to serine, threonine side chains) in proteins. This type of post-translational modification greatly affects cellular trafficking, compartmentalization, and membrane tethering. Palmityltransferase (PAT), the enzyme responsible for this modification transfers a palmitate moiety from palmitoyl-CoA to the thiol group of cysteine in the target protein. Compared to myristoylation, palmitoylation is reversible and the reverse reaction is catalyzed by thioesterases. The Palmitoylation/Depalmitoylation cycle plays an important role when modified protein shuttles between cellular compartments. PAT mutations are associated with many neurological diseases and cancer progression. BioVision offers EZClick™ Palmitoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of palmitoylated proteins. The kit uses a modified Palmitic Acid that is fed directly into the cells and gets incorporated into proteins during or post translation. This post translational modification can be followed by click reaction with an azide-containing dye. The assay kit offers a powerful method for imaging localization, trafficking, and dynamics of Palmitoylated proteins, or detection by FACS for quantitative studies. Labeled Palmitoylated Proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.

Alternate Name: EZClick™ Palmitoylated Protein Assay (FACS/Microscopy), Green Fluorescence, EZClick™ Palmitoylated Protein Kit (FACS/Microscopy), Green Fluorescence, Palmitoylated Protein Assay Kit, EZClick™ Palmitoylated Protein Assay Kit, Green, EZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence, EZClick™ Palmitoylated Protein Kit (FACS/Microscopy), Green, EZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), EZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Green, Palmitoylated Protein Assay

Tag Line: for labeling and detection of Palmitoylated proteins

Summary: BioVision offers EZClick™ Palmitoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of palmitoylated proteins

Detection Method: Fluorescence Microscope Imaging and FACS analysis

Sample Type: Suspension or adherent cell cultures

Species Reactivity: Mammalian

Applications: Detection and quantification of biosynthesis, dynamics and turnover of palmytoylated proteins, Screening for genotoxic compounds and effectors of protein modifications, Identification and localization of palmitic acid modified proteins

Features & Benefits: Highly specific, Robust method for labeling and detection of Palmitoylated proteins, Simple procedure