- Home
- Biovision Assay Kits
- EZClick™
- EZClick™ Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
Biovision
EZClick™ Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
- SKU:
- 26-K453
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 100 Assays
- Storage Conditions:
- -20°C
- Shipping Conditions:
- Gel Pack
- Shelf life:
- 12 months
Description
Stearoylation is one of the numerous types of post-translational modification in proteins. Stearoylated proteins are involved in regulation of cellular processes such as apoptosis, growth and metabolism. In this modification, Stearic acid, a saturated fatty acid, is covalently bound to a protein through the action of long-chain-fatty-acid-[acyl-carrier-protein] ligase. In the presence of ATP, this enzyme transfers a Stearate moiety to the target protein forming a carbon-sulfur bond (C=S). Recent studies showed a possible correlation between mitochondrial dysfunction with some neurodegenerative diseases, cancer and aging. Furthermore, it is known that stearoylated Transferrin Receptor (TfR1) inhibits JNK activation, reduces mitofusin ubiquitination, thus restoring mitochondrial function. Studying of stearoylation of proteins can shed a light for disease etiology and prevention. BioVision offers EZClick™ Stearoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of Stearoylated proteins. The kit uses Alkynyl Stearic Acid that is fed directly into the cells and gets incorporated into proteins during or post translation. This modification can be followed by click reaction with an azide-containing dye. The assay kit offers a powerful method for imaging localization, trafficking, and dynamics of Stearoylated proteins, or detection by FACS for quantitative studies. Labeled Stearoylated Proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.
Alternate Name: EZClick™ Stearoylated Protein Assay (FACS/Microscopy), EZClick™ Stearoylated Protein Assay Kit, EZClick™ Stearoylated Protein Kit (FACS/Microscopy), Stearoylated Protein Assay Kit, EZClick™ Stearoylated Protein Assay Kit (FACS/Microscopy)
Tag Line: for labeling and detection of Stearoylated proteins
Summary: BioVision offers EZClick™ Stearoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of Stearoylated proteins.
Detection Method: Fluorescence Microscope Imaging and FACS analysis
Sample Type: Suspension or adherent cell cultures
Species Reactivity: Mammalian
Applications: Identification and localization of stearic acid modified proteins, Screening for genotoxic compounds and effectors of protein modifications, Detection and quantification of biosynthesis, dynamics and turnover of stearic acid modified proteins
Features & Benefits: Robust method for labeling and detection of Stearoylated proteins, Highly specific, Simple procedure
Related Products

QuickDetect™ Alpha-enolase (ENO1) (Human) ELISA Kit
Biovision

StemBoost™ Growth Factor Set X
Biovision

ExoPure™ Immunoplates (Overall Exosome Isolation, serum, colorimetric assay)
Biovision

Human CellExp™ SARS-CoV-2 Spike RBD (L452R, T478K), Recombinant
Biovision
Customers Also Viewed

Hi-Bind™ T-Gel-Agarose
Biovision

RNAkeep™ Solution
Biovision

Hi-Bind™ Cibacron Blue-Agarose Beads
Biovision

Hi-Bind™ Ni QR Agarose Beads
Biovision

Hi-Bind™ Protein G-Agarose
Biovision

EZSolution™ABT-494 (Upadacitinib)
Biovision

Tyloxapol, MegaPure™ Detergent, (10%) solution
Biovision

EZScreen™ ATP Colorimetric Assay Kit (384-well)
Biovision

EZScreen™ Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit (384-Well)
Biovision

EZQuant™ RNA Quantification Kit II
Biovision

EZQuant™ RNA Quantification Kit I
Biovision

EZQuant™ dsDNA Quantitation Kit (Fluorometric)
Biovision

EZLabel™ Protein FITC Labeling Kit
Biovision

EZLabel™ Protein Cy5 Labeling Kit
Biovision

EZLabel™ Protein Cy3 Labeling Kit
Biovision

EZLabel™ Protein Biotin Labeling Kit
Biovision

EZClick™ Sialic Acid (ManAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
Biovision

EZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence
Biovision

EZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
Biovision

Human CellExp™ Contactin-6 (Fc Tag), Human Recombinant
Biovision

Human CellExp™ Contactin-5 (His Tag), Human Recombinant
Biovision

Human CellExp™ Contactin-1 (His Tag), Human Recombinant
Biovision

Human CellExp™ IL-2 Receptor Alpha, Human Recombinant
Biovision

Human CellExp™ FOLR1, Fc Tag, Human Recombinant
Biovision

Human CellExp™ CD19, Fc Tag, Human recombinant
Biovision

Human CellExp™ ROR2, Fc Tag, Human Recombinant
Biovision

Human CellExp™ ROR1, Fc Tag, Human Recombinant
Biovision

Human CellExp™ TDGF1, Fc Tag, human recombinant
Biovision

RNaseRIP™ Solution
Biovision

StemBoost™ Growth Factor Set VII
Biovision

StemBoost™ Growth Factor Set VI
Biovision

EZCell™ Protein Transport Inhibitor Cocktail (500X)
Biovision

EZCell™ Cell Stimulation and Protein Transport Inhibitor Cocktail (500X)
Biovision


EZCell™ Cell Stimulation Cocktail (500X)
Biovision

Anti-MMP3 recMAb™ Antibody
Biovision

QuickDetect™ Leptin (Human) ELISA Kit
Biovision

QuickDetect™ INF-alpha (Human) ELISA Kit
Biovision

QuickDetect™ IL-6 (Human) ELISA Kit
Biovision

QuickDetect™ IL-13 (Human) ELISA Kit
Biovision

QuickDetect™ IgM (Rat) ELISA Kit
Biovision

QuickDetect™ IgM (Rabbit) ELISA Kit
Biovision

QuickDetect™ IgM (Mouse) ELISA Kit
Biovision

QuickDetect™ IgM (Human) ELISA Kit
Biovision

QuickDetect™ CD28 (Human) ELISA Kit
Biovision

QuickDetect™ CD163 (Human) ELISA Kit
Biovision

QuickDetect™ CD14 (Human) ELISA Kit
Biovision

QuickDetect™ Catecholamine (CA) (Rat) ELISA Kit
Biovision

QuickDetect™ Catecholamine (CA) (Mouse) ELISA Kit
Biovision

QuickDetect™ beta-Endorphin (Rat) ELISA Kit
Biovision

QuickDetect™ beta-Endorphin (Mouse) ELISA Kit
Biovision