EZClick™ TUNEL – in situ DNA Fragmentation/Apoptosis Assay Kit

Apoptosis is a controlled phenomenon in which apoptotic cells are characterized by changes in nucleus (i.e. chromatin condensation, DNA fragmentation), cytoplasm shrinkage and production of apoptotic bodies. These are subsequently phagocytosed by macrophages, parenchymal cells, or neoplastic cells. DNA strand breaks can be analyzed by TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End-Labeling) assays. BioVision’s EZClickTM TUNEL-DNA fragmentation/Apoptosis Assay utilizes modified EdUTP nucleotides which are incorporated at the 3’-OH ends of fragmented DNA by Terminal deoxynucleotidyl transferase (TdT) enzyme and detected based on a click reaction. This assay enables easy delivery and incorporation of EdUTP coupled with high selectivity of detection of DNA damage. The assay is performed under mild reaction conditions which preserve cell morphology, thus enabling the identification of damaged DNA by FACS or fluorescence microscopy. Our assay is fast and capable of detecting a higher percentage of apoptotic cells than the antibody based methods. The kit contains sufficient reagents to detect total/fragmented DNA in apoptotic cells in a 1 X 96-well plate or on 50 cover slips.

Alternate Name: TUNEL assay

Tag Line: Non-antibody based assay for the detection of apoptotic cells

Summary: N/A

Detection Method: Flow cytometer w/lasers capable of excitation at 488 and 530/590 nm emission filters respectively

Sample Type: Cell culture

Species Reactivity: All

Applications: Detection of DNA fragmentation during apoptosis and programmed cell death,Evaluating effects of anti-cancer drugs and genotoxic agents on DNA structure,Screening for genotoxic compounds and effectors of DNA integrity and cellular changes,In-situ studies of apoptosis and programmed cell death

Features & Benefits: Does not require antibodies, avoiding lengthy incubation times and does not require washes,Specific, sensitive,Compatible with Flow Cytometry and Fluorescence Microscopy instruments