PicoProbe™ 15-PGDH Activity Assay Kit (Fluorometric)

15-Hydroxy prostaglandin dehydrogenase (15-PGDH; EC:1.1.1.141) is a rate-limiting enzyme for prostaglandin catabolism. It oxidizes prostaglandins (PG) to 15-keto metabolites. In general, 15-keto metabolites show reduced biological activity, when compared to PG. PGs are generated from arachidonic acid by cyclooxygenase (COX), and play an important role in inflammatory responses. Recent study found that 15-PGDH serves as a degrading enzyme for PG and serves as a tumor suppressor to inhibit cancer progression. Thus, study of 15-PGDH activity can elucidate and clarify unknown aspects related to cancer development and therapeutics. In BioVision’s 15-PGDH Assay Kit, 15-PGDH oxidizes a substrate forming intermediates and NADH. The oxidation of NADH reduces a probe generating fluorescence at Ex/Em=535/587 nm. The activity of 15-PGDH is proportional to the fluorescent signal. BioVision’s 15-PGDH Activity Assay Kit offers a rapid, simple, sensitive, and reliable test suitable for detecting as low as 1 µU of 15-PGDH activity

Alternate Name: N/A

Tag Line: Highly Sensitive Assay

Summary: • Detection method- Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian • Application- The 15-PGDH fluorometric assay kit is a highly sensitive assay and can detect < 5 µU 12-PGDH activity (based on our unit definition) in a variety of samples.

Detection Method: Fluorescence (Ex/Em 535/587 nm)

Sample Type: • Animal tissues: liver, kidney, lung etc. • Cell culture: adherent or suspension cells

Species Reactivity: Eukaryotes

Applications: • Measurement of 15-PGDH activity in various tissues/cells. • Analysis of prostaglandin catabolism in various cell types.

Features & Benefits: • Simple procedure • Fast and convenient • Sensitive assay for measuring 15-PGDH activity in various biological samples and the kit is also suitable for high throughput analyses.