PicoProbe™ Malate Dehydrogenase Activity Assay Kit (Fluorometric)

Malate Dehydrogenase (MDH) (EC 1.1.1.37) reversibly converts L-Malate into oxaloacetate in the presence of NAD+. In eukaryotic cells, malate dehydrogenase has 2 isoforms, MDH1 and MDH2. MDH1 is cytosolic and participates in the malate-aspartate shuttle, which transports malate into the mitochondria. Malate will then be utilized for the generation of ATP. MDH2, on the other hand, is a mitochondrial enzyme that participates in the citric acid cycle. MDH2 activity is increased in some neurodegenerative diseases such as Alzheimer’s disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma). Biovision’s PicoProbeTM Malate Dehydrogenase Activity Assay Kit (Fluorometric) provides a quick and easy method for monitoring MDH activities in a wide variety of samples. In this assay, MDH converts malate into intermediate products, which will further react with PicoProbeTM forming a fluorophore (Ex/Em=535/587nm). The kit is simple, sensitive and high-throughput adaptable and can detect as low as 40 µU of MDH activity.

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Summary: • Detection method- Fluorescence(535/587 nm) • Applications- - Measurement of malate dehydrogenase activity in various tissues/cells - Analysis of citric acid cycle and malate-asparate shuttle

Detection Method: Fluorescence (535/587 nm)

Sample Type: • Animal tissues such as liver, heart, muscle, etc. • Cell culture: Adherent or suspension cells • Mitochondria

Species Reactivity: Mammalian

Applications: • Measurement of malate dehydrogenase activity in various tissues/cells • Analysis of citric acid cycle and malate-asparate shuttle

Features & Benefits: • Simple, rapid & convenient • The assay can detect Malate Dehydrogenase (MDH) Activity less than 100 µU in various sample types.