PicoProbe™ β-Hydroxybutyrate Fluorometric Assay Kit

β-Hydroxybutyrate (β-HB) is the most abundant of the ketone bodies (~78% of total ketone bodies in blood). Ketoacidosis (high concentrations of the ketone bodies) can be caused by a variety of conditions, such as diabetes, alcoholism and severe starvation. Ketoacidosis can be fatal if not treated. Additionally, ketogenic diets can increase the concentration of ketone bodies slightly in healthy individuals. BioVision’s β-Hydroxybutyrate Assay Kit offers simplicity, enhanced sensitivity, and can be adapted to high-throughput applications. The assay is based on enzymatic oxidation of β-HB that results in generation of fluorescent signal (Ex/Em = 535/587 nm) which is directly proportional to the amount of β-HB. The kit offers an excellent alternative for measurement of β-HB when biological sample quantities are limited or subketogenic levels are suspected. The assay can detect β-Hydroxybutyrate as low as 4 µM in a variety of biological samples.

Alternate Name: PicoProbe™ 3-Hydroxybutyrate Fluorometric Assay Kit

Tag Line: The most sensitive kit on the market

Summary: • Detection method- Fluorescence (Ex/Em = 535/587 nm) • Applications: - Measurement of β-Hydroxybutyrate in various biological samples - Analysis of β-Hydroxybutyrate in pathological conditions

Detection Method: Fluorescence (Ex/Em = 535/587 nm)

Sample Type: • Serum, plasma • Urine or other body fluids • Food such as milk etc.

Species Reactivity: N/A

Applications: • Measurement of β-Hydroxybutyrate in various biological samples • Analysis of β-Hydroxybutyrate in pathological conditions

Features & Benefits: • Simple, rapid & high-throughput adaptable • Highly sensitive: The assay can detect less than 4 µM of β-Hydroxybutyrate (β-HB) in various sample types.