PicoProbe™ myo-Inositol Assay Kit (Fluorometric)

Inositol is a carbocyclic sugar that is also known as vitamin B8 and is believed to have numerous health benefits. This substance must be obtained either from exogenous sources, or generated from metabolism of glucose-6-phosphate. While several isomers exist, myo-Inositol is the only biologically relevant form. When metabolized, it becomes a central component of multiple secondary signaling molecules, including inositol phosphates and phosphatidylinositol. These compounds participate in a number of eukaryotic signaling pathways, from insulin signaling and gene expression to cytoskeletal assembly and intracellular calcium mediation. BioVision’s PicoProbe myo-Inositol Assay Kit gives the user a specific, rapid, high-throughput adaptable method for determination of this molecule in biological samples. A Sample Clean-Up Mix is provided for easy removal of glucose in the sample that may contribute to background. Our assay will quantify amounts of inositol as low as 20 pmoles.

Alternate Name: N/A

Tag Line: Highly Sensitive; validated

Summary: • Detection method- Fluorescence (535/587 nm) • Applications-Detects inositol amounts as low as 20 pmol.

Detection Method: Fluorescence (Ex/Em 535/587 nm)

Sample Type: • Biological Fluids (Breast Milk, Saliva, Plasma or Serum) • Tissue or Cell Lysate

Species Reactivity: Eukaryotes

Applications: Measurement of myo-Inositol content in liquid samples and from cell and tissue lysates

Features & Benefits: • Simple procedure; takes ~ 60 minutes • Fast and convenient • The kit provides an accurate assay for measuring inositol in various samples