PicoProbe™ NADPH Quantitation Fluorometric Assay Kit

NADP (nicotinamide dinucleotide) is a major electron donor for anabolic reactions such as lipid and nucleic acid synthesis. The reduced form, NADPH is a cofactor for many redox enzymes. It is of increasing interest to be able to measure low level of intracellular NADPH. BioVision’s PicoProbe™ NADPH Quantitation Assay kit provides a highly sensitive method to detect intracellular NADPH. NADH is not detected as the NADPH Recycling Enzyme Mix specifically recognizes NADPH in the enzyme recycling reaction. The assay is rapid, simple and does not require purifying NADPH from samples. The kit can measure less than 40 nM NADPH in a variety of samples.

Alternate Name: PicoProbe™ Nicotinamide adenine dinucleotide phosphate Quantitation Fluorometric Assay Kit

Tag Line: The most sensitive kit on the market

Summary: • Detection method- Fluorescence (Ex/Em = 535/587 nm) • Applications: - Measurement of NADPH in various tissues/cells - Analysis of metabolism and cell signaling in various cells

Detection Method: Fluorescence (Ex/Em = 535/587 nm)

Sample Type: • Animal tissues: Liver, muscle, heart etc. • Cell culture: Adherent or suspension cells • Mitochondria

Species Reactivity: N/A

Applications: • Measurement of NADPH in various tissues/cells • Analysis of metabolism and cell signaling in various cells

Features & Benefits: • Simple, rapid & convenient • The kit can measure less than 40 nM NADPH in a variety of samples