PicoProbe™ Proline Assay Kit (Fluorometric)

Proline is a proteogenic amino acid, which plays an important role in protein folding. In humans, proline can be synthesized from glutamate and arginine. Proline is one of the conditionally essential amino acids in humans. Proline is also produced by honey bees as they process nectar into honey. Thus, its content in honey is often used as an indicator of honey ripeness and sugar adulteration. For mature honey, 180 mg-proline/kg of honey is used as an international standard. Proline contents are measured for its biological importance in plants where it also serves as a stress marker. Unstressed plants contain approximately 0.5 µmol proline per gram of plant tissue while stressed plants can range up to 100 times more proline than the unstressed ones. BioVision’s PicoProbe™ Proline Assay Kit provides a quick, specific and easy method for measuring proline concentrations in a wide variety of samples. In this assay, proline is converted to an intermediate, which will further react with a probe to produce a strong fluorometric signal (Ex/Em = 535/587 nm). The kit is simple, easy to use, sensitive and high-throughput adaptable. It can detect as low as 5 pmol of proline per well.

Alternate Name: PicoProbe™ Proline Kit (Fluorometric), Proline Kit (Fluorometric), Proline Fluorometric Assay Kit, Proline Kit, Proline Assay Kit (Fluorometric), PicoProbe™ Proline Assay Kit, PicoProbe Proline Assay Kit (Fluorometric), Proline Fluorometric Assay, Proline Kit (Fluorometric), PicoProbe™ Proline Assay Kit (Fluorometric), Proline Assay Kit, PicoProbe™ Proline Assay Kit, Proline Assay Kit (Fluorometric), Proline Assay, Proline Fluorometric Kit, Proline Fluorometric Assay

Tag Line: for measuring proline concentrations

Summary: BioVision’s PicoProbe™ Proline Assay Kit provides a quick, specific and easy method for measuring proline concentrations in a wide variety of samples. In this assay, proline is converted to an intermediate, which will further react with a probe to produce a strong fluorometric signal (Ex/Em = 535/587 nm). The kit is simple, easy to use, sensitive and high-throughput adaptable. It can detect as low as 5 pmol of proline per well.

Detection Method: Fluorescence (Ex/Em = 535/587 nm)

Sample Type: Mammalian and plant tissue, Biological fluids: serum, honey, etc.

Species Reactivity: All

Applications: Assaying proline concentration as a stress factor in plants., Analyzing maturation of honey., Measurement of proline in biological samples and honey.

Features & Benefits: Specific, Easy method for measuring Proline concentrations in a wide variety of samples., Quick