PicoProbe™ Reduced Glutathione (GSH) Assay Kit (Fluorometric)

Glutathione (GSH), a thiol-containing tripeptide, is a key antioxidant in living systems. Intracellular GSH status appears to be a sensitive indicator of the overall health of a cell. BioVision’s PicoProbeTM Reduced Glutathione Assay Kit is based on a specific enzymatic cycling method in the presence of GSH and a fluorophore. The reduction of the fluorophore produces a stable fluorescent product, the fluorescence of which is directly proportional to the amount of GSH in the sample and can be followed kinetically (Ex/Em=535/587 nm). Our assay is the most specific and sensitive in the market. Oxidized Glutathione does not interfere with the assay. The assay is simple, reproducible and can specifically detect as low as 8 pmol/well of reduced form of Glutathione (GSH) in a 100 µl reaction

Alternate Name: N/A

Tag Line: It specifically detects reduced glutathione. Oxidized GSSG is not detected.

Summary: • Detection method- Fluorescence (535/587 nm) • Species reactivity- Mammalian • Applications- The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.

Detection Method: Detection method- Fluorescence (Ex/Em 535/587 nm)

Sample Type: Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Species Reactivity: Mammalian

Applications: The Fluorometric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.

Features & Benefits: • Simple procedure; takes only ~1-2 hours • Fast and convenient • Specific: does not detect oxidized GSSG • Sensitive: detects as low as 8 pmol GSH