QuickDetect™ 24(S)-Hydroxycholesterol (Human) ELISA Kit

An important cholesterol metabolite (cholesterol 24-hydroxylase) found in the brain. It is an endogenous agonist of the nuclear receptor LXR. It has been shown to induce cell death in neuroblastoma cells, possibly via a necroptosis pathway. 24(S)-Hydroxycholesterol has also been found to be a high affinity ligand for RORα and RORγ (Ki = 25 nM). 24(S)-Hydroxycholesterol levels are elevated in the cerebral spinal fluids of patients with neurogenerative diseases suggesting possible clinical applications. It was also able to reduce Aβ production and increase ER-resident immature amyloid precursor protein (APP) levels in human neuroblastoma SH-Sy5Y cells and CHO cells via inhibition of intracellular amyloid precursor protein trafficking. BioVision’s QuickDetect™ 24-OHC ELISA kit is a sandwich ELISA assay for measuring the quantity of human 24-OHC in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results.

Alternate Name: N/A

Tag Line: A rapid sandwich ELISA kit for the quantitative measurement of CD8 in human serum, plasma, cell culture supernatnat and other biological fluids

Summary: • Detection method- Absorbance (450 nm) • Species reactivity- Human • Application- quantitative measurement of 24-OHC in human serum, plasma, cell culture supernatant, and other biological fluid.

Detection Method: Absorbance (450 nm)

Sample Type: Human serum, plasma, cell culture supernatant, tissue homogenate and other biological fluids.

Species Reactivity: Human

Applications: This ELISA kit is used for quantitative measurement of 24-OHC in human serum, plasma, cell culture supernatant and other biological fluids.

Features & Benefits: Easy, convenient and time-saving method to measure the level of 24-OHC in human serum, plasma, culture supernatants and other biological fluids. The entire assay can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results. Detection Range: 0.5 -100 ng/ml