ADPsensor™ Universal Kinase Activity Assay Kit

Protein Kinases belong to a large family of phosphotransferases that mediate a wide spectrum of metabolic events via signal transduction pathways. Protein Kinases catalyze transfer of a phosphate group from a phosphate donor to a substrate protein. Kinases modulate the function of numerous proteins and are well-known therapeutic targets in many diseases like cancer, inflammation and diabetes etc. BioVision’s ADPsensor™ Universal Kinase Assay Kit is based on the fluorescence detection of ADP generated by Kinase activity. In this assay, ADP formed during the kinase reaction is converted to an intermediate by the ADP Sensor Mix, which then reacts with a probe to generate a strong and stable fluorescence signal at Ex/Em = 535/587 nm. ATP in the kinase reactions doesn’t interfere with the assay; therefore our kinase assay can be read kinetically (or at endpoint). The kit provides a universal tool to measure kinase activity in presence of substrates of your interest, or to screen/characterize kinase inhibitors. The assay is simple, non-radioactive, highly sensitive (<0.05 µM ADP) with broad ATP tolerance (up to 1 mM). The kit can detect as low as 1 ng of active Kinase.

Alternate Name: N/A

Tag Line: Fast, sensitive, High-throughput compatible, fluorometric Kinase assay with detection limit as low as 1 ng of active Kinase.

Summary: Ultrasensitive, Simple, HTP adaptable fluorometric assay kit for Kinase activity measurement. Can detect as low as 1 ng of active Kinase. Detection method- Fluorometric (Ex/Em = 535/587 nm). Applications- Detection of Kinase activity, Characterization of Kinase substrates and inhibitors. Sample Type: Purified/Enriched Kinases, Tissue or cell extracts.

Detection Method: Fluorometric (Ex/Em = 535/587 nm)

Sample Type: • Purified/semi-purified/immuno-precipitated Kinases • Crude cell/tissue extract

Species Reactivity: Universal

Applications: Detection of Kinase activity, Characterization of Kinase substrates and inhibitors.

Features & Benefits: • Ultrasensitive, Simple, HTP adaptable fluorometric assay kit for Kinase activity measurement. • Can detect as low as 1 ng of active Kinase. • Free of ATP interference (up to 1 mM)