EZSolution™ Benzo-Endonuclease

Biomolecule/Target: N/A

Synonyms: Serratia marcescens binuclease; unrestricted endonuclease

Alternates names: Serratia marcescens binuclease; unrestricted endonuclease

Taglines: For digestion of DNA and RNA

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: N/A

NCBI Gene Symbol: N/A

Gene Source: N/A

Accession #: N/A

Recombinant: Produced and purified from eukaryotic yeast cells

Source: Serratia marcescens gene

Purity by SDS-PAGE #: ≥90%

Assay: SDS PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: None

Activity (Specifications/test method): ≥ 1.0 x 106 KU/mg

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit (U) of Binuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min under the condition above, which means complete digestion of 37 μg DNA

Molecular Weight: 30 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Liquid

Reconstitution Instructions: N/A

Background Information: Benzo-Endonuclease is a genetically engineered non-specific endonuclease from Serratia marcescens gene. Functionally, this promiscuous nuclease digests all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5’ monophosphate terminated oligonucleotides 2-5 bases in length. Benzo-Endonuclease is not a sequence dependent nuclease, capable of cleaving the phosphodiester bond at all positions among a nucleic acid chain. The enzyme is produced and purified from eukaryotic yeast cells, without contamination of endotoxin from prokaryotic cells and is highly stable even under harsh industrial conditions. It has no detectable proteinase or esterase activity. Benzo-Endonuclease is an ideal tool enzyme in protein purification and DNA/RNA contamination from purified proteins

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans