EZCut™ TEV Protease, Recombinant
- Usually Shipped in 5 Working Days
- Storage Temperature:
- Shipping Conditions:
- dry ice
- Shelf Life:
- 12 months
Biomolecule/Target: TEV Protease
Synonyms: Nuclear inclusion protein A, NIa protein
Alternates names: Nuclear inclusion protein A, NIa protein
Taglines: ≥95% Pure active, highly site specific and stable recombinant TEV Protease
Country of Animal Origin: USA
NCBI Gene ID #.: N/A
NCBI Gene Symbol: N/A
Gene Source: N/A
Accession #: N/A
Source: E. coli
Purity by SDS-PAGE #: ≥95%
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): The EZCut™ TEV Protease has an activity of ≥10,000 units/mg.
Biological activity: The EZCut™ TEV Protease has an activity of ≥10,000 units/mg.
Results: ≥10,000 units/mg
Binding Capacity: N/A
Unit Definition: One unit is defined as the amount of EZCut™ TEV Protease required to cleave >90% of 10 µg a control protein substrate (containing an ENLYFQS sequence at N-terminus) in 1 h at 37°C.
Molecular Weight: 28.6 kDa (2038–2279 aa + C-terminal poly-his tag).
Concentration: 1 mg/ml
Physical form description: 1 mg/ml solution in 0.1 M Tris-HCl, 0.5 M NaCl, 20% glycerol, 5 mM DTT and 0.5 mM EDTA, pH 8.0
Reconstitution Instructions: N/A
Background Information: BioVision’s EZCut™ TEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). It contains an enhanced form of a catalytic fragment of the NIa protein of Tobacco etch virus (TEV). TEV Protease is a restriction grade protease that has robust activity at 4°C with high specificity and great stability. The optimal temperature for cleavage with this enzyme is 34°C. The protease can be used for the removal of affinity tags from fusion proteins. It contains a C-terminal His tag and can be easily removed after cleavage reactions by passing the reaction through a Ni-chelating resin. BioVision’s EZCut™ TEV Protease is an improved version of TEV protease that is highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in enhanced long-term activity.
Amino acid sequence: 2038–2279 aa
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans