EZCut™ TEV Protease, Recombinant

Biomolecule/Target: TEV Protease

Synonyms: Nuclear inclusion protein A, NIa protein

Alternates names: Nuclear inclusion protein A, NIa protein

Taglines: ≥95% Pure active, highly site specific and stable recombinant TEV Protease

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: N/A

NCBI Gene Symbol: N/A

Gene Source: N/A

Accession #: N/A

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): The EZCut™ TEV Protease has an activity of ≥10,000 units/mg.

Biological activity: The EZCut™ TEV Protease has an activity of ≥10,000 units/mg.

Results: ≥10,000 units/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of EZCut™ TEV Protease required to cleave >90% of 10 µg a control protein substrate (containing an ENLYFQS sequence at N-terminus) in 1 h at 37°C.

Molecular Weight: 28.6 kDa (2038–2279 aa + C-terminal poly-his tag).

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 0.1 M Tris-HCl, 0.5 M NaCl, 20% glycerol, 5 mM DTT and 0.5 mM EDTA, pH 8.0

Reconstitution Instructions: N/A

Background Information: BioVision’s EZCut™ TEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). It contains an enhanced form of a catalytic fragment of the NIa protein of Tobacco etch virus (TEV). TEV Protease is a restriction grade protease that has robust activity at 4°C with high specificity and great stability. The optimal temperature for cleavage with this enzyme is 34°C. The protease can be used for the removal of affinity tags from fusion proteins. It contains a C-terminal His tag and can be easily removed after cleavage reactions by passing the reaction through a Ni-chelating resin. BioVision’s EZCut™ TEV Protease is an improved version of TEV protease that is highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in enhanced long-term activity.

Amino acid sequence: 2038–2279 aa

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans