EZLys™ Lysozyme, Human

Biomolecule/Target: N/A

Synonyms: Protein Extraction

Alternates names: Lysozyme, EZLys™ , 1,4-beta-N-acetylmuramidase C

Taglines: An enzyme which breaks down bacterial cell wall

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: N/A

NCBI Gene Symbol: N/A

Gene Source: N/A

Accession #: N/A

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGE #: ≥85%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: >100,000 units/mg protein. A unit (U) is defined as the amount of enzyme which will reduce the absorbance at 450 nm, using a suspension of Micrococcus lysodeikticus as a substrate in a 2.6 ml reaction volume, by 0.001 absorbance units per min at pH 6.24 and 25°C.

Molecular Weight: N/A

Concentration: N/A

Appearance: Off- white powder

Physical form description: Off- white powder

Reconstitution Instructions: Clear solution at 10 mg/ml in PBS, pH 7.4 or H₂O

Background Information: EZLys™ Lysozyme is a recombinant human lysozyme expressed and purified in an animal-free system (rice). It has an identical amino acid sequence and physic-chemical properties of native lysozyme. The enzyme cleaves b (1,4) linkage between N-acetylglucosamine and N-acetylmuramic acid of the peptidoglycan component in the bacterial cell wall. It exhibits four times more activity than commercial chicken lysozyme and is useful in the recovery of proteins expressed in bacteria (e.g. E. coli). EZ Lysozyme is designed for gentle cell lysis which leads to a minimal risk of protein denaturation.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans