Human CellExp™ B7-1/CD80, Human Recombinant

Biomolecule/Target: N/A

Synonyms: T-lymphocyte activation antigen CD80; B7-1; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7

Alternates names: T-lymphocyte activation antigen CD80; B7-1; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7

Taglines: A critical regulator of T- and B-cell responses

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 941

NCBI Gene Symbol: N/A

Gene Source: Human

Accession #: P33681

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE #: > 95% SDS-PAGE

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Human biotinylated CD28, Fc Tag (BioVision Cat. No. 9237) can bind to immobilized human CD80, Fc Tag at 1 µg/mL, 100 µL/well with a linear range of 0.01 – 0.27 µg/mL

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 50 kDa, calculated (with human Fc Tag fused to C-terminus)

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstitute in sterile PBS, pH 7.4. Do not vortex.

Background Information: B-lymphocyte activation antigen B7, also known as B7-1 and Cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 and CD86 (B7-2), together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to CD80 and CD86 with a 20 to 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. CD80 works in tandem with CD86 to prime T cells and plays a role in induction of innate immune responses by activating NF-κB signaling pathway in macrophages. Additionally, the up-regulation of CD80 has been linked to various autoimmune diseases, including multiple sclerosis, systemic lupus erythematosus and sepsis. CD80 is thus regarded as a promising therapeutic target for autoimmune diseases and various carcinomas.

Amino acid sequence: Val 35 - Asn 242, with C-terminal human Fc tag

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans