Human CellExp™ B7-1 / CD80, mouse recombinant
- Usually Shipped in 5 Working Days
- Storage Temperature:
- Shipping Conditions:
- Gel pack
- Shelf Life:
- 12 months
Synonyms: CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
Alternates names: CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
Taglines: Plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages
Country of Animal Origin: USA
NCBI Gene ID #.: 12519
NCBI Gene Symbol: Cd80
Gene Source: Mouse
Accession #: Q00609
Source: HEK 293 cells
Purity by SDS-PAGE #: >95%
Assay #2: N/A
Endotoxin Level: < 1 EU/μg
Activity (Specifications/test method): Measured by its binding ability in a functional ELISA
Biological activity: Immobilized Mouse B7-1, His Tag at 2μg/Ml (100 μL/well) can bind Mouse CTLA-4, Fc Tag with a linear range of 0.12-0.5 ng/mL.
Binding Capacity: Immobilized Mouse B7-1, His Tag at 2μg/Ml (100 μL/well) can bind Mouse CTLA-4, Fc Tag with a linear range of 0.12-0.5 ng/mL.
Unit Definition: N/A
Molecular Weight: 25.6 kDa
Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally Mannitol or Trehalose is added as a protectant before lyophilization.
Reconstitution Instructions: Reconstitute in sterile deionized water to a concentration of 50 μg/ml.
Background Information: B7-1 and B7-2, together with their receptors CD28 and CTLA4, constitute one of the dominant co-stimulatory pathways that regulate T and Bcell responses. Although both CTLA4 and CD28 can bind to the same ligands, CTLA4 binds to B71 and B72 with a 20 100 fold higher affinity than CD28 and is involved in the downregulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA- 4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans