Biovision
Human CellExp™ Cathepsin B, human recombinant
- SKU:
- 26-7408
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 μg
- Storage Temperature:
- -20°C
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Description
Biomolecule/Target: Cathepsin B
Synonyms: CTSB, CPSB, APPS
Alternates names: CTSB, CPSB, APPS
Taglines: Participates in intracellular degradation and turnover of proteins
Taglines: USA
Country of Animal Origin: USA
NCBI Gene ID #.: 1508
NCBI Gene Symbol: CTSB
Gene Source: Human
Accession #: P07858
Recombinant: Yes
Source: HEK293 cells
Purity by SDS-PAGE #: ≥95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: <1 EU/μg by LAL method
Activity (Specifications/test method): N/A
Biological activity: Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100 μl reaction mixture containing 25 mM MES, pH 5.0, 0.01 µg rhCathepsin B, and 10 µM reaction substrate. The specific activity is >2800 pmol/min/µg.
Results: Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100 μl reaction mixture containing 25 mM MES, pH 5.0, 0.01 µg rhCathepsin B, and 10 µM reaction substrate. The specific activity is >2800 pmol/min/µg.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with a 6×His tag at the C-terminus, has a calculated MW of 36.7 kDa (Pro) and 29 kDa (Mature). The predicted N-terminus is Arg18 (pro) or Phe74 (mature). DTT-reduced Protein migrates as 43 kDa and 34 kDa due to glycosylation.
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM Tris-HCl and 150 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Background Information: Cathepsin B (CTSB) also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans