Human CellExp™ CD25/IL-2R alpha, C-His Tag, Human Recombinant

Biomolecule/Target: IL-2R

Synonyms: IL2RA, Interleukin-2 receptor subunit alpha, p55, CD25, IL2-RA, TAC antigen,

Alternates names: IL2RA, Interleukin-2 receptor subunit alpha, p55, CD25, IL2-RA, TAC antigen,

Taglines: Extracellular Domain of IL-2R fused to 6x His tag at the C-terminus.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 3559

NCBI Gene Symbol: IL2RA

Gene Source: Human

Accession #: P01589

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused to 6x His tag at the C-terminus and has a calculated MW of 26 kDa. The protein behaves as ~55 kDa and ~100 kDa polypeptide under reducing (R) and non-reducing (NR) conditions (SDS-PAGE) due to glycosylation and dimerization.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from 0.22 μm filtered PBS (pH 7.4) with 5% trehalose.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Background Information: Interleukin-2 receptor subunit alpha (IL2RA) is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells. IL2RA binds IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covalently with the IL2 receptor beta and gamma chain, and subsequently initiates intracellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been considered as an activation marker. While both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans