Human CellExp™ CD47, Fc Tag, Mouse Recombinant

Biomolecule/Target: N/A

Synonyms: CD47, MER6, IAP, OA3

Alternates names: CD47, MER6, IAP, OA3

Taglines: Plays a role in cell adhesion modulation of integrins

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 16423

NCBI Gene Symbol: Cd47

Gene Source: Murine

Accession #: Q61735

Recombinant: FALSE

Source: HEK 293 cells

Purity by SDS-PAGE #: >95% as determined by SDS-PAGE

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 0.1 EU per μg by the LAL method.

Activity (Specifications/test method): Immobilized Mouse CD47, Mouse IgG2a Fc Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human SIRP alpha, Fc Tag with a linear range of 0.16-5 μg/mL

Biological activity: N/A

Results: N/A

Binding Capacity: Immobilized Mouse CD47, Mouse IgG2a Fc Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human SIRP alpha, Fc Tag with a linear range of 0.16-5 μg/mL

Unit Definition: N/A

Molecular Weight: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.7 kDa. The protein migrates as 55-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 µg/ml. Do not vortex. It is recommended to store at -20°C.

Background Information: Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

Amino acid sequence: AA Gln 19 - Lys 140

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans