Human CellExp™ CD47, Rhesus macaque Recombinant

Biomolecule/Target: CD47

Synonyms: CD47, MER6, IAP, OA3

Alternates names: CD47, MER6, IAP, OA3

Taglines: Plays a role in cell adhesion modulation of integrins

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 704980

NCBI Gene Symbol: CD47

Gene Source: Rhesus macaque

Accession #: F7A802

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: > 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/μg

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Immobilized Human SIRP alpha, Fc Tag at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque CD47, His Tag with a linear range of 4-63 ng/mL

Results: N/A

Binding Capacity: Immobilized Human SIRP alpha, Fc Tag at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque CD47, His Tag with a linear range of 4-63 ng/mL

Unit Definition: N/A

Molecular Weight: 15.8 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Generally Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to a concentration of 50 µg/ml.

Background Information: Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans