Human CellExp™ CNTN2/Contactin-2, human recombinant

Biomolecule/Target: CNTN2/Contactin-2

Synonyms: CNTN2, CNTN-2, AXT, DKFZp781D102, FLJ37193, FLJ42746, MGC157722, TAG-1, TAG1, TAX, TAX1, TAX-1, Contactin-2

Alternates names: CNTN2, CNTN-2, AXT, DKFZp781D102, FLJ37193, FLJ42746, MGC157722, TAG-1, TAG1, TAX, TAX1, TAX-1, Contactin-2

Taglines: Mediates cell-cell interactions either via homophilic, or heterophilic contacts with various adhesion molecules.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 6900

NCBI Gene Symbol: CNTN2

Gene Source: Human

Accession #: Q02246

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been precoated with 50 μl/well of the rhContactin2 solution at 8-30 μg/ml.

Results: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been precoated with 50 μl/well of the rhContactin2 solution at 8-30 μg/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×his tag at the C-terminus and has a calculated MW of 109 kDa expressed. The predicted N-terminus is Ser 31. Protein migrates as 140 kDa in reduced SDS-PAGE resulting from glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Contactin-2 also known as CNTN2, TAX1 (transiently-expressed axonal glycoprotein), TAG1 (transient axonal glycoprotein), and axonin-1, and is a member of the immunoglobulin superfamily. CNTN2 consists of six Ig-like domains and four fibronectin type III domains, and is anchored to the membrane by glycosylphosphatidylinositol (GPI), whereas the soluble form can be released by a GPI-specific phospholipase. As a neural cell adhesion molecule expressed by a subset of neuronal populations in the developing CNS and PNS, CNTN2 mediates cell-cell interactions either via homophilic, or heterophilic contacts with various adhesion molecules including NgCAM, NrCAM, NCAM and neurocan. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. It may also be involved in glial tumorigenesis and may provide a potential target for therapeutic intervention.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans