Human CellExp™ EGFR/ErbB1, human recombinant

Biomolecule/Target: ErbB1

Synonyms: EGFR, ERBB, ERBB1, HER1, PIG61, mENA

Alternates names: EGFR, ERBB, ERBB1, HER1, PIG61, mENA

Taglines: A cell-surface receptor for members of the EGF-family of extracellular protein ligands.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 1956

NCBI Gene Symbol: EGFR

Gene Source: Human

Accession #: P00533

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized human EGF at 10 μg/ml can bind human EGFR with a linear range of 0.5–750 ng/ml.

Results: Measured by its binding ability in a functional ELISA. Immobilized human EGF at 10 μg/ml can bind human EGFR with a linear range of 0.5–750 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 69.5 kDa. The predicted N-terminus is Leu 25. DTT-reduced Protein migrates as 110-115 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans