Human CellExp™ Fc gamma RIIIB /CD16b, human recombinant

Biomolecule/Target: Fc gamma RIIIB /CD16b

Synonyms: FCGR3B, CD16B, FCG3B, FCGR3, FCG3, IGFR3

Alternates names: FCGR3B, CD16B, FCG3B, FCGR3, FCG3, IGFR3

Taglines: Involved in activation of the NK cells for Antibody-Dependent Cell-mediated Cytotoxicity (ADCC)

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 2215

NCBI Gene Symbol: FCGR3B

Gene Source: Human

Accession #: O75015

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA.

Results: N/A

Binding Capacity: Recombinant Human Fcγ RIIIB / CD16b can bind immobilized Anti-CD20 (Rituximab Cat. No. A1049) coated at 2 μg/ml (100 μl/well)) with a linear range of 1 - 50 μg/ml

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 22 kDa. The predicted N-terminus is Gly 17. DTT-reduced Protein migrates as 38-50 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered PBS (pH 7.4) with 5% trehalose.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.

Background Information: CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner. CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16b, also known as FCGR3B and FCG3B, is expressed specifically by neutrophils and stimulated eosinophils. CD16b is the low affinity receptor for the Fc region of immunoglobulins gamma. It binds complexed or aggregated IgG and also monomeric IgG. CD16b by itself is not capable of mediating antibody-dependent cytotoxicity and phagocytosis and cannot transduce signals from outside to inside the cell but may do so by associating with cell-specific components. Soluble CD16b has a regulatory role in inflammatory processes and induces the production of pro-inflammatory cytokines.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans