Human CellExp™ GM-CSF, Human Recombinant

Biomolecule/Target: N/A

Synonyms: Human Cellexp Human Recombinant GM-CSF

Alternates names: CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897.

Taglines: A glycoprotein, growth factor and cytokine that stimulates the bone marrow to produce granulocytes and stem cells

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 1437

NCBI Gene Symbol: CSF2

Gene Source: Human

Accession #: P04141

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 μg of protein

Activity (Specifications/test method): N/A

Biological activity: ED₅₀ is typically 0.4 to 2 ng/mL. The specific activity was determined by the dose- Dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic Indicator cell line).

Results: 0.4 to 2 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 15 to 36 kDa, monomer, glycosylated

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from a PBS solution.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free, recombinant human serum albumin.

Background Information: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a growth factor that supports the in-vitro colony formation of granulocytes-macrophages progenitor cells. It is a pleiotropic cytokine and is produced as a response to immune or inflammatory stimuli by activated cells of the hematopoietic system such as T cells, B cells, macrophages, mast cells, fibroblasts and alveolar epithelial cells. It plays an important role in regulating the proliferation, differentiation, survival and activation of hematopoietic cells such as granulocytes and monocytes, neutrophiles, basophiles and eosonophoiles, erythroid cells, megakaryocytes and T cells. Human and mouse GM-CSF have about 56% homology and are species specific. Human GM-CSF is not active on mouse cells and vice versa. It is active on canine and feline cells.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans