Human CellExp™ M-CSF, Human Recombinant
- Usually Shipped in 5 Working Days
- Storage Temperature:
- Shipping Conditions:
- gel pack
- Shelf Life:
- 12 months
Synonyms: Human Cellexp Human Recombinant M-CSF
Alternates names: Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.
Taglines: A hematopoietic growth factor
Country of Animal Origin: USA
NCBI Gene ID #.: 1435
NCBI Gene Symbol: CSF1
Gene Source: Human
Accession #: P09603
Source: Human 293 cell expressed
Purity by SDS-PAGE #: ≥95%
Assay #2: N/A
Endotoxin Level: < 1.0 EU per 1 μg of protein
Activity (Specifications/test method): N/A
Biological activity: The EC50 is typically 0.5 to 4 ng/mL for this protein with specific activity determined by the dose dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line)
Results: 0.5 to 4 ng/ml
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein has 223 amino acids (Glu 33 - Arg 255) with a 6x-His tag at C-terminus, and a calculated MW of 26 kDa. The protein migrates to 35-45 kDa under reducing and non-reducing conditions (SDS-PAGE) due to glycosylation
Physical form description: Lyophilized from 0.22 μm filtered PBS (pH 7.4) with 5% trehalose.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.
Background Information: Granulocyte/Macrophage Colony-Stimulating Factors (M-CSF) are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of granulocytes and monocytes-macrophages. M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. It also plays an important role in innate immunity and in inflammatory processes by promoting the release of proinflammatory chemokines. M-CSF is also required for normal bone development and male/female fertility.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans