Human CellExp™ Her2/ErbB2, Extracellular Domain (ED), Human Recombinant

Biomolecule/Target: ERBB2

Synonyms: erbB-2, MNL 19, CD340, Neu, c-ErbB-2, NEU, NGL, TKR1

Alternates names: erbB-2, MNL 19, CD340, Neu, c-ErbB-2, NEU, NGL, TKR1

Taglines: Involved in the signal transduction pathways leading to cell growth and differentiation.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 2064

NCBI Gene Symbol: ERBB2

Gene Source: Human

Accession #: P04626

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: > 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Immobilized Human Her2 (ED) at 0.05 μg/ml (100 μl/well) can bind trastuzumab (Herceptin) with a linear range of 0.05-3 ng/ml.

Results: N/A

Binding Capacity: Immobilized Human Her2 (ED) at 0.05 μg/ml (100 μl/well) can bind trastuzumab (Herceptin) with a linear range of 0.05-3 ng/ml.

Unit Definition: N/A

Molecular Weight: 70.6 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS pH 7.4. Generally 5-8% Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to a concentration up to 0.2 mg/ml.

Background Information: Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2, HER-2 /neu, NEU, NGL, TKR1 and c-erb B2, and is a protein involved in higher aggressiveness of breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans