Human CellExp™ HER3 / ErbB3 Extracellular Domain (ED), Human Recombinant

Biomolecule/Target: ErbB3

Synonyms: ERBB3, ErbB-3, HER3, HER-3, LCCS2, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3, Receptor tyrosine-protein kinase erbB-3

Alternates names: ERBB3, ErbB-3, HER3, HER-3, LCCS2, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3, Receptor tyrosine-protein kinase erbB-3

Taglines: Involved in pathways leading to cell proliferation or differentiation.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 2065

NCBI Gene Symbol: ErbB3

Gene Source: Human

Accession #: P21860

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: HER3-biotin can bind immmobilized Human Her2 (ED) (Cat. No. P1160 at 5 μg/ml (100 μl/well)) with a linear range of 0.05-5 μg/ml.

Results: HER3-biotin can bind immmobilized Human Her2 (ED) (Cat. No. P1160 at 5 μg/ml (100 μl/well)) with a linear range of 0.05-5 μg/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus and has a calculated MW of ~70 kDa (20 – 643 aa). Under reducing conditions the protein migrates to ~93 kDa in SDS-PAGE due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered PBS (pH 7.4)

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in distilled water to a concentration up to 0.2 mg/ml.

Background Information: ErbB3, also called human epidermal growth factor receptor 3 (HER3), is a type I membrane glycoprotein and a member of the ErbB family. ErbB family members serve as receptors for the epidermal growth factors (EGF). ErbB3 is expressed in normal adult human gastrointestinal tract, reproductive system, skin, nervous system, urinary tract, and endocrine system. ErbB3 is the only member with a defective kinase domain among ErbB family members. However, ErbB3 can form heterodimers with ErbB2 to complement a high affinity receptor complex. ErbB3 has been shown to bind the ligands heregulin and NRG-2. The lethal congenital contracture syndrome 2 (LCCS2) disease is caused by mutations affecting ErbB3. ErbB3 null mouse embryos show severely underdeveloped atrioventricular valves, leading to death at embryonic day 13.5.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans