Human CellExp™ IL-2 R beta /CD122, human recombinant

Biomolecule/Target: IL-2 R beta /CD122

Synonyms: IL2RB, RP5-1170K4.6, CD122, P70-75

Alternates names: IL2RB, RP5-1170K4.6, CD122, P70-75

Taglines: Involved in receptor mediated endocytosis

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 3560

NCBI Gene Symbol: IL2RB

Gene Source: Human

Accession #: P14784

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.5 - 2.5 µg/mL in the presence of 4.0 ng/mL of recombinant human IL-15.

Results: Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.5 - 2.5 µg/mL in the presence of 4.0 ng/mL of recombinant human IL-15.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.4 kDa. The predicted N-terminus is Ala 27. DTT-reduced Protein migrates as 40 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to a cytokine called IL-2. The IL-2R is made up of 3 subunits - α (CD25), β (CD122) and γ (CD132) - non-covalently associating. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ-chain, along with the β subunit. CD122 also known as IL2R beta, is a member of the type I cytokine receptor family. CD122 is the receptor for interleukin-2 and is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans